Research Technician

Hillman Cancer Center seeks a qualified research technician to assist in transplant and leukemia immunotherapy research in Dr. Sawa Ito's laboratory. The program aims to perform immunological correlating studies for the clinical trial and understand the treatment resistance mechanism. The technician is expected to perform human sample processing, experiments, data collection, and analysis of cellular, immunological, and molecular assays, including flow cytometry, primary cell and cell line culture, cell line development, DNA/RNA/protein preparation, RT-PCR and DNA/RNA sequencing, and T cell receptor (TCR) transduction to the human primary cells. The optimal candidate should have at least a Bachelor's degree with 3 years of experience or a Master's degree with 1 year of experience. Experience or training in either molecular biology or immunology is preferred.

We aim to conduct extensive correlating studies, including flow-based immunophenotyping assays and single-cell RNAseq, to assess leukemia IFN-gamma responsiveness. In addition to this new funding, we have an ongoing project to characterize the T cell receptor (TCR) clones of alloreactive T cells and discover the novel antigens recognized by these alloreactive TCRs. We seek a Research technician in our lab to accomplish these new and ongoing projects.

Performs a variety of independent and complex laboratory work and experiments. Analyzes and interprets data, compiles results, and prepares reports. Maintains inventory, ordering, and logs. Writes manuscripts and grant proposals and prepares data visualizations.

Candidate must be able to independently perform advanced scientific experiments. Must be highly organized and detail oriented.

Moderate physical effort required. May be required to stand/move for several hours at a time. May be required to move objects up to 50 pounds with or without assistance of equipment.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.