Staff Research Associate 1 - Cell Culture

The Staff Research Associate will support Lawrence Lab (Lawrence-lab.org) research in the areas of cell culture and cell signaling, and with experience will have the opportunity to contribute to research projects in the lab and to author publications. Responsibilities include cell culture of mammalian cell lines including human cancer cell lines and human induced pluripotent stem cells (iPSCs), as well as iPSC-derived astrocytes. The work will also involve basic molecular biology approaches such as cloning of plasmids, preparation of buffers and solutions, RT-PCR, immunostaining, RNA FISH, immunostaining and immunoblotting, and the keeping of weekly summaries of experiments and cell lines. The work will also involve live cell imaging, the generation of modified induced pluripotent stem cell lines and genomics analyses such as DNA methylation mapping. The incumbent will be required to coordinate and work together as a team with undergraduate and graduate students, postdocs, and project scientists. Experience in cell culture. particularly human pluripotent stem cell cultures, methods like RNA FISH and imaging, are essential. The incumbent will work with the lab personnel on other tasks as needed.

Salary:$26.52 - $31.63 hourly

REQUIRED

• Previous experience working in a laboratory setting and conducting research.
  
• Working skills with basic molecular biology laboratory equipment.
  
• Ability to perform several tasks at once, communicate clearly and work in a team environment. Ability to learn new methods and protocols.
  
• Must be responsible and able to work independently, without close supervision.
  
• Knowledge of PC and macintosh computers, spreadsheet and word processing programs.
  
• Ability to perform basic technical duties such as logging in reagents and data analyses.
  
• High organizational skills; Ability to organize lab stocks and keep careful records in lab databases and lab notebooks.
  

PREFERRED

• BS in Molecular Biology or related field.
  
• Knowledge of cell culture, including human pluripotent stem cell cultures, genotyping, and immunofluorescence is highly valuable.
  
• General knowledge of cell and molecular biology.
  
• Previous experience in managing basic laboratory safety protocols highly desirable.