Job Title: Scientist
Location: South San Francisco, CA, 94080
Duration: 12 Months (possible extension)
Position Summary: Perform hands-on CHO cell line development and characterization studies to advance novel biotherapeutics into human clinical studies.
Key Responsibilities:
- Perform CHO cell line development, including transfection, selection, single cell subcloning, growth and productivity screening, cell banking and stability assessment of cell banks.
- Design and generate DNA vector constructs for protein (including monoclonal antibodies and antibody-like molecules).
- Culture mammalian cells across multiple scales and formats, from 96-well plates up to shake flasks.
- Operate instruments like ViCell automated cell counter, Octet, Clone Select Imager, microscope, HPLC, ambr15, Beacon and flow cytometer. Share responsibility for equipment maintenance.
- Perform genetic characterization of cell banks using molecular techniques like DNA sequencing and PCR.
- Review DNA sequencing data (including NGS), Southern/Northern blots, quantitative PCR results.
- Author and review cell line development technical reports that meet expectations of regulatory agencies for all stages from IND to BLA.
- Present updates at cross-functional meetings and collaborate closely with cell culture, purification, analytical and formulation development colleagues to meet project timelines.
- Actively seek opportunities to optimize or enhance the cell line development workflow.
- May publish or present scientific findings in peer-reviewed journals, internal or external conferences or consortia.
- Display effective organization, verbal and written communication skills, and adherence to good documentation practices.
Qualifications & Experience:
- Scientist I: BS with a minimum of 5 years or MS with a minimum of 3 years of relevant experience.
- Scientist II: BS with a minimum of 7 years or MS with a minimum of 5 years of relevant experience.
- Proficient in aseptic techniques, mammalian cell culture and basic molecular biology techniques (molecular cloning, plasmid preps, DNA digests/gels, PCR/qPCR).
- Experienced in developing stably transfected CHO cell lines for production of monoclonal antibodies and antibody-like molecules.
- Strong understanding of mammalian cell biology and molecular biology concepts.
- Must have excellent attention to detail and must keep detailed records in electronic lab notebook on a daily basis.
- Demonstrated ability to independently design, execute and interpret critical experiments to answer scientific questions.
- Demonstrated ability to learn, understand and master new experimental techniques.
- Demonstrated ability to work collaboratively within and outside of the group.
- Demonstrated ability to author technical reports and presentations.
- Proficient with Microsoft Office software and able to learn new software applications.
- Able to multi-task and work within timelines.
- Strong communication skills - verbal, written, scientific data presentation.
Preferred:
- Background in CHO cell line development for GMP applications within biotech/pharma industry.
- Understanding of cell biology and bioprocess engineering concepts.
- Hands-on experience running RNA-seq workflow and analysis.
- Experience in bioinformatics software to analyze DNA and RNA for sequence confirmation and expression analysis.
- Proficiency in programming/data science languages, such as R, Python, and Java.
- Experience with flow cytometry.
- Experience with ddPCR.
- Experience with ambr15 operation.
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Spectraforce Technologies
Aug 02, 2025