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Research Specialist

Johns Hopkins University
United States, Maryland, Baltimore
Nov 15, 2024

We are seeking a Research Specialist that is a driven individual to join a laboratory that uses mouse models of bone marrow transplantation (BMT) to study the epigenetic mechanisms contributing to the development of graft-versus-host disease (GVHD) and the graft-versus-leukemia (GVL) effect.

Specific Duties & Responsibilities

  • Conduct murine BMT experiments using established protocols that include,
    • Harvest of murine spleen for single cell suspension.
    • Splenic T-cell purificatio.
    • Splenic T-cell flow cytometry.
    • Harvest of murine bone marrow.
    • Animal irradiation.
    • Tail vein injections.
  • Participate in long-term monitoring of experiments, including examination of animals and GVHD scoring using an established clinical scale.
  • Assist with analyzing and interpreting murine GVHD and survival data.
  • Participate in post-transplant analyses, including the procurement of organs for fixation, and single-cell suspensions for surface and intracellular multiparameter flow cytometry staining of lymphoid and myeloid cells.
  • Analyze and interpret flow cytometry data.
  • Collaborate with the PI to oversee scientific management of the laboratory.
  • Collaborate with PI or other lab members on research projects
  • Identify and select best options for scientific strategies and research development based on literature reviews and collaboration with colleagues.
  • Perform a range of laboratory assays.
  • Contribute to writing of scientific publications and grant applications.
  • Act as a resource for laboratory techniques for post-doctoral fellow and students and junior technicians.


Minimum Qualifications
  • Bachelor's Degree in Biology, Chemistry or related field.
  • One year laboratory experience performing relevant techniques.
  • Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.

Classified Title: Research Specialist
Job Posting Title (Working Title):Research Specialist
Role/Level/Range: ACRP/03/MA
Starting Salary Range: $16.92 - $29.61 HRLY (Commensurate with experience)
Employee group: Full Time
Schedule: M-F, 830-500
Exempt Status:Non-Exempt
Location:School of Medicine - East Baltimore Campus
Department name: 10002936-SOM Onc Pediatric Oncology
Personnel area: School of Medicine

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