Laboratory Neuroscientist Technician

22nd Century Technologies, Inc.
Pay Rate Range: $65K-$70K/
United States, Maryland, Rockville
Sep 27, 2024
Job Title: Laboratory Neuroscientist Technician Location: Rockville MD 20852 Duration: 02 Years to Long term Shift: Standard Shift (Mon to Fri) 40 Hours/Week Teleworking Eligible: Yes Overall Position Summary and Objectives: Developing an integrated clinical, basic, and translational research ecosystem that identifies and characterizes immunologic processes that causally contribute to psychiatric illness, starting with rare autoantibodies in neuropsychiatric disorders. Min Education: Bachelor's Skills: Experience in CLIA-certified lab. Experience in rodent handling and husbandry. Expertise in cell line and primary cell culture. Expertise in small rodent sacrifice, perfusion, cryopreservation, and paraffin embedding. Expertise in immunostaining: immunofluorescence, DAB, and colorimetric. Animal Handling: Non-human primates. Software: Power point. MS Project. MS Access. Field of Study: Microbiology. Molecular Biology. Certifications & Licenses Clinical Laboratory Improvement Amendments (CLIA). Statement of Work Details: Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research. Draft and maintain standard operating procedures for antibody screens and testing. Operates laboratory machines and instruments; and prepares specimens and equipment for use. Maintain electronic biospecimen handling and testing logs Performs sample management and storage. Schedule regular maintenance of equipment logs and maintain performance logs Analyzes, documents and reports on all procedures performed. Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results Maintains inventory of laboratory supplies. Cleans and sterilizes instruments, glassware and equipment. Immunostain rodent and/or NHP brain tissue with human CSF, serum/plasma, and commercial antibodies Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research. Preparation of presentation slides and figures for outside investigators . Occasionally attend lab meetings of outside investigators Maintains inventory of laboratory supplies and initiates purchase orders. Assist in troubleshooting and calibrating various laboratory equipment and instruments. Collect, analyze and interpret data; prepare summaries and reports based on results and findings Performs routine equipment maintenance. Primary cell isolation and culture (neurons, astrocytes, oligodendrocytes, and mixed cell culture) . iPSC-derived neuronal cell differentiation and culture. Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research. Perform experiments on samples according to protocols. Logging of assay and experimental results in electronic laboratory notebook (Lab Archives) Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff. Human protein array immunostaining (HuProt) and data processing. Performs advanced equipment maintenance. screening for autoantibodies in human biofluids (blood fractions and cerebrospinal fluid) using a variety of methods including rodent(and non-human primate) brain tissue immunostaining, human protein array, iPSC-derived neuronal culture and immunostaining, and (less commonly) immunoprecipitation mass spectrometry (IP-MS). Trains staff in performing routine laboratory tests and procedures. Expected to engage with outside investigators for the purpose of interpreting and contextualizing preliminary results Provides insights and expertise and recommends new strategies and approaches. collaborate with in-house bio informaticists, biologists, and biochemists and have opportunities to mentor junior scientists contribute to the development of secondary neuroscientific questions stemming from antibody discovery research - present work at national conferences and publish their findings in peer-reviewed journals.