Cell Culture Specialist I
Meso Scale Diagnostics LLC | |
United States, Maryland, Gaithersburg | |
9168 Gaither Road (Show on map) | |
Oct 09, 2024 | |
Description
POSITION SUMMARY: Under the direction of the manager, this position is responsible for the production of cell-based products including but not limited to: hybridoma antibodies and recombinant antibodies. Primarily responsible for cell culture and managing multiple purifications systems. DUTIES AND RESPONSIBILITIES: * Manipulate multiple cell lines (both adherent and suspension), such as mouse hybridoma cell , Expi 293 and Expi CHO cells for the production of monoclonal antibodies and other cell based products. * Execute transfections for the production of recombinant QRM antibodies. * Use the following cell culture equipment: Hemocytometer, Biological Safety Cabinet, Automated Cell Counter, Plate Readers (for ELISA and BCA assays), LN2 Freezers, Humidified Incubators, Water Baths, CO2 Tanks, and Controlled Rate Freezer. * Use AKTA purification systems * Provide general cell culture laboratory maintenance. Keep process areas stocked with essential equipment and supplies and maintain overall laboratory appearance and cleanliness. * Coordinate regular equipment maintenance and calibrations; complete and document equipment validation as required in MFG. * Perform daily scheduled cell culture and purification processes to meet production demand * Perform vialing and storage of cell based products. * Maintain and organize inventory of supplies, including but not limited to cell stocks and records, to support daily production. * Other duties as assigned. EXPERIENCE AND QUALIFICATIONS: * Bachelor's degree in Biology, Chemistry, Biotechnology, Engineering or a related field or equivalent applicable work experience. May substitute degree for equivalent combination of experience/education. * Minimum of three months of experience performing cell culture using aseptic technique in a laboratory setting required. * Experience in a GMP/GLP, ISO Certified environment preferred. KNOWLEDGE, SKILLS AND ABILITIES: * Knowledge of manufacturing mammalian cell based products preferred * Knowledge of Hybridoma cell culture and expansion preferred. * Knowledge of common cell culture equipment: Hemocytometer, Biological Safety Cabinet, Automated Cell Counter, Plate Readers (for ELISA and BCA assays), LN2 Freezers, Humidified Incubators, Water Baths, CO2 Tanks, and Controlled Rate Freezer. * Proficiency in MS Office Suite and SharePoint preferred. * Attention to detail with demonstrated commitment to excellence and performance * Organizational, planning, and time management skills with the ability to manage multiple and often changing priorities while meeting time-sensitive deadlines and deliverables. * Good communication skills with the ability to organize, present, and articulate ideas both verbally and in writing. * Basic knowledge of aseptic technique; ability to complete cell culture in the absence of antibiotics. * Ability to work both independently and as an effective team member. * Willingness and ability to acquire new skills in various cell culture methods as necessary. * Ability to understand and follow safety procedures. * Ability to move/lift up to 40 pounds. PHYSICAL DEMANDS: * While performing the duties of this job, the individual is frequently required to sit and stand for long periods. The individual is occasionally required to walk; use hands and fingers to operate, handle, or feel objects, tools, or controls; reach with hands and arms. * May be required to move/lift up to 40 pounds. WORK ENVIRONMENT: * This position is performed in a traditional industrial laboratory environment but can also include work in a laboratory environment (which can include work with chemicals and hazardous materials. Education
Bachelors of Biology (preferred)
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