Histology Specialist Immunopathology

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• Assists in the overseeing of the Immunofluorescence laboratory of the Immunopathology Unit.
• Trains new technologists on proper procedures and techniques, evaluates new technology for clinical application and assists in updating procedure manuals for laboratory inspections and accreditations such JCAHO.
• Functions as principal technical specialist for the Immunofluorescence laboratory, provides instruction in special techniques, methodology and the reading and interpreting of test results to students, residents; provides all of the above except the reading and interpreting of test results to the pathologists.
• Performs diagnostic direct immunofluorescence staining procedures on skin and kidney biopsies and examines slides with pathologist using a fluorescence microscope for the detection of various immune-mediated disease processes.
• Performs indirect immunofluorescence staining procedures using patient sera for the detection of autoantibodies in patients suspected of having immuno-mediated disease of the skin and the kidney. Documents results by photographing and maintaining log books.
• Performs gel electrophoresis, transfer, and Western Blot Analysis for the detection of anti-GBM antibodies for vacation and sick coverage.
• Performs enzyme linked immunosorbent assays (ELISA) for detection of autoantibodies directed against the glomerular basement membrane (anti-GBM).
• Maintains quality control logs on a variety of equipment including cryostat, microtome, centrifuge, refrigerators etc.
• Picks up clinical diagnostic biopsies as needed, including dividing specimen appropriately for electron microscopy, immunofluorescence and light microscopic studies.
• Accessions specimens and bills patients for all clinical services.
• Maintains inventory and orders supplies as needed.
• Responsible for quality control of reagents including antibodies.

• Minimum of B.S. or B.A. degree in Biology or Chemistry or equivalent
• 3-5 years of laboratory experience
• ASCP Certification Required

• Familiarity with immunology, computers, and the metric system. Knowledge of chemistry; familiarity with the concepts of molarity, normality, molecular weight, formula weight of chemicals.
• Familiarity with how to make dilutions from stock solutions.
• Knowledge of good laboratory technique.
• Must be able to do mathematical calculations, and read/write English.
• Must know proper handling procedure of chemicals.
• Effective interpersonal and communication skills, attention to detail.
• Must be organized and capable of following protocols and have ability to devise or adapt techniques if needed to achieve desired goals.
• Supervisory skills are needed to instruct technologist or residents.
• Ability to recognize novel or emergency situations and respond appropriately.
• Good hand and eye coordination and manual dexterity.
• Ability to work well with others.

• May be exposed to disease-bearing specimens, hazardous chemicals or fumes, other hazards normally associated with a clinical laboratory setting.
• Work environment may be noisy or crowded.

• None