Senior Scientist in Proteomics - 137068

The UCSD Department of Pharmacology is one of two basic science units in the School of Medicine where world-class researchers investigate the effects of chemical interventions on biological systems. The faculty are internationally renowned for their strength in elucidating basic cellular and molecular mechanisms of signal transduction that lead to innovative drug target identification and ultimately to cutting edge drug discovery. The faculty also play a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines, and provide diversity to meet the interests of a broad spectrum of students and scholars. More than one hundred trainees at the graduate student and postdoctoral level, as well as a staff of more than 50 research technicians and department administrators interact closely with the faculty. The diverse mix of ages, backgrounds, and talents creates a robust work environment with challenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically-advanced and interested individuals to become a part of our dynamic exploratory research and educational environment. For more information, please visit our website at http://pharmacology.ucsd.edu.

Under direction and in consultation with the Principal Investigator, the incumbent will perform a variety of experimental procedures for ongoing laboratory research projects to study bacterial pathogenesis, host responses to infection, and the human microbiome. The incumbent will develop and apply cutting-edge quantitative proteomics and metabolomics tools to track thousands of protein dynamics for over 1300 clinical stool samples. The incumbent will then interface microbiology techniques to characterize important factors identified using proteomics. Based on previous proteomics data, the researcher will synthesize a chimeric protein to develop a vaccine against Group A Strep (GAS). Research will utilize in vitro and in vivo techniques including microbiology, cloning, protein expression, mass spectrometry procedures, in vivo murine studies testing vaccine candidates and maintaining all data analysis using the associated software. Independently investigate techniques that may not be standard for the lab, and adapt them for use in the lab, write protocols, and lead and train other staff personnel and students in their use. Independently recognize problems and identify solutions, contribute original ideas, analyze data and present findings in meetings and complete written reports. This position is afforded significant latitude for complex research projects, decision-making and independent judgment. Incumbent will contribute content for the preparation of laboratory manuscripts for publication and grant applications and will assist the PI with laboratory management to include; EH&S compliance, maintenance of key laboratory equipment/machines, and ordering supplies).

• Extensive theoretical knowledge of chemistry, biochemistry, cloning, and computer science as typically attained by a Bachelor's degree, and/or an equivalent combination of education and experience.

• Proven ability to contribute to original project development ideas, experimental design and strategies, as well as their implementation into a variety of research projects.

• Demonstrated knowledge of microbiology techniques and procedures in reference to working with various chemicals including acid, and bacteria, especially pathogenic strains, including liquid-liquid extractions, culturing, and genetic manipulation. Ability to prepare and maintain media buffers and mathematical skills to perform molar calculations.

• Skilled in the operation, maintenance, and calibration of laboratory equipment including: centrifuges, pH meters, spectrophotometers, balances, computers, etc.

• Knowledge of qPCR thermocycler instrumentation, operation, and maintenance for Eppendorf RealPlex 4.

• Advanced knowledge of mass spectrometry instrumentation, operation, and maintenance for Dionex Ultimate 3000 HPLC Required G Advanced knowledge in the application of softwares including Ardia, Proteome Discoverer, and Cytoscape.

• Advanced knowledge in the application of softwares including Chromeleon, Xcalibur, Proteome Discoverer, and Cytoscape.

• Excellent interpersonal skills. Ability to communicate verbally and in writing to a diverse population including persons from foreign countries, the business community, academics, students and staff with a high degree of diplomacy, mature judgment, tact and flexibility.

• Demonstrated ability to effectively document complex research procedures, accurately record experimental details and results, perform mathematical calculations necessary for data analysis, prepare graphs and figures to summarize results and write reports.

• Ability to utilize multiple types of computer programs for performance of scientific equipment, recording of data, data analyses, statistical evaluation, graphical presentation of results, written descriptions of research, and illustrations of research topics.

• Ability to work independently, take ownership of work performance and follow through on assignments. Anticipate, identify, and analyze situations and independently seek solutions or recommend course of action as appropriate. Excellent time management and strong demonstrated organizational and multitasking skills including establish priorities, meet multiple and frequently changing deadlines with flexibility, process a high volume of work while under pressure and maintain a high level of accuracy.

• Ability and experience troubleshooting and recommending modifications to procedures and protocols to meet the needs of research projects. Ability to analyze and interpret results.

• Ability to direct, advise, and train other lab personnel on procedures, methods, and data analysis, as well as explain difficult concepts and safety issues involved in a wide variety of scientific experimentation.

• Ability to prepare, interpret and present the results of scientific experiments in the pursuit of specific research goals and for the publication of experimental results in peer reviewed scientific journals.

• Knowledge of EH&S compliance and Injury and Illness Prevention Program. Knowledge of laboratory safety precautions and proper handling of chemicals & biohazardous materials, including appropriate clothing and shoes and use of other protective items.

• Employment is subject to a criminal background check.

• Must be willing to work a flexible schedule to accommodate protein purification. Work in cold room (4 degrees C).