Junior Specialist - Aghi Lab

Junior Specialist - Aghi Lab

The Aghi Lab is seeking Junior Specialists who will participate in basic science research to study mechanisms by which brain tumors interact with their microenvironment to promote growth, invasion, and therapeutic resistance. Drug(s) to disrupt these processes will be evaluated in collaboration with industry partners in cell culture and in mouse models. Job duties will include culturing tumor cells; extraction of RNA and DNA form brain tumor tissue; cloning retroviral constructs and isolating and purifying retroviruses from infected cells; immunohistochemistry and immunofluorescence of tumor tissue and cultured primary tumor cells; isolation of cells from brain tumors and flow cytometry of the cells to count numbers of various cell types; isolation of stem cells from brain tumors; CRISPR techniques; and intracranial and subcutaneous implantation of tumor cells into mice, followed by treatment and monitoring of these animals and euthanasia when appropriate. The candidates will also handle lab administration - order entry, making sure protocols are in compliance, and orienting new lab members. Other duties as assigned.

Required Qualifications:

• Specialists appointed at the junior rank must possess (or in process of obtaining) a baccalaureate degree (or equivalent degree) or must have at least four years of research experience.
• Applicants must have obtained the degree requirement for the Specialist rank by the time of hire.
• Experience with basic wet lab research (cell culture, pipetting, sectioning tissue, immunostaining, qPCR, mouse tumor implantation, western blotting).
• Ability to learn more advanced wet lab techniques (CRISPR, immunoprecipitation, purification of histidine-tagged proteins).
• Ability to independently plan experiments from start to finish to address a specific topic, ultimately leading the person filling this position to write portions of manuscripts.
• Ability to handle lab administration, including order entry, making sure protocols are in compliance, and orienting new lab members.
• Applicant's materials must list qualifications upon submission.

Preferred Qualifications:

• Independent specialized research.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $55,000 - $58,600.

Please apply online at https://aprecruit.ucsf.edu/JPF05908.

• 3 required (contact information only)

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

UCSF is committed to welcoming and serving all people, honoring the dignity of every individual without preference or prejudice, in support of its public mission and in alignment with our PRIDE values and Principles of Community.

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.

* "Misconduct" means any violation of the policies or laws governing conduct at the applicant's previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, or discrimination, as defined by the employer.

* UC Sexual Violence and Sexual Harassment Policy

* UC Anti-Discrimination Policy

* APM - 035: Affirmative Action and Nondiscrimination in Employment