Research Assistant I - Schlaeger Lab

• Produce, maintain, and conduct quality control on human pluripotent stem cells.
• Perform cellular analysis using techniques such as immunostaining, flow cytometry, DNA/RNA isolation, PCR, and other molecular biology methods.
• Operate and maintain laboratory instruments, including flow cytometers, automated fluorescence microscopes, plate washers, plate readers, and liquid handling robotics.
• Manage laboratory inventory by monitoring, ordering, and organizing supplies.
• Prepare media and stock solutions for ongoing experiments.
• Ensure strict compliance with institutional and federal guidelines regarding health, safety, and animal use regulations.
• Develop research figures and assist in writing and updating laboratory protocols.
• Mentor junior lab members and instruct them on proper laboratory techniques.
• Present research findings and progress updates during weekly lab meetings.

• Bachelor's degree in Biological Sciences, Biochemistry, Chemistry, or a closely related field required; Master's degree preferred.

• Extensive hands-on undergraduate or graduate research laboratory experience highly preferred.
• Willingness to work with animal models and human tissue samples.
• Excellent organizational skills and the ability to multi-task effectively to meet project deadlines.
• Proficiency in following multi-step protocols and standard operating procedures (SOPs).
• Ability to work both independently and collaboratively within a goal-focused team environment.
• Commitment to a two-year duration in this role.
• Human pluripotent stem cell culture and manipulation experience preferred.
• CRISPR genome editing experience preferred.
• Standard molecular biology techniques experience preferred.
• Chemical and high-throughput screening methods, including the use of robotics experience preferred.
• ddPCR and FACS (Fluorescence-Activated Cell Sorting) experience preferred.
• Confocal microscopy and advanced image analysis experience preferred.
• cGMP regulations and tissue culture practices experience preferred.

The posted pay range is Boston Children's reasonable and good-faith expectation for this pay at the time of posting.

Any base pay offer provided depends on skills, experience, education, certifications, and a variety of other job-related factors. Base pay is one part of a comprehensive benefits package that includes flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.