Senior Research Technologist- Mouse Microsurgeon

Department: MED-Thoracic Surgery
Salary/Grade: NEX/15

Target hiring range for this position will be between $32.84-$41.05 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.

Job Summary:

Conducts complex experimental research in a research lab, functioning as a specialist or technical exConducts complex experimental research in a research lab, functioning as a specialist or technical expert. May manage one or more phase(s) of a major project(s) or all activities of a small project. May ensure overall lab compliance with all associated rules and regulations.

We are seeking a highly skilled and extensive experienced Senior Research Lab Technician specializing in mouse microsurgery to join our research team. This position will be responsible for performing intricate surgical procedures on laboratory mice to support our research project in immunology of transplantation field. The successful candidate will have a strong background in mouse handling, anesthesia administration, and surgical techniques, such as lung transplant, spleen transplant.

The training for these procedures can be provided but prior experience with these techniques is highly desirable.

The successful candidate should be able to work independently as well as part of a team. and also, could conduct moderately complex experiments under the supervision of a junior scientist. Our lab is a collaborative group with investigators from Thoracic Surgery, Pulmonary & Critical Care Medicine, Rheumatology, and Immunology. For more information on our work, please refer to Bharat Lab's publications.

This role will have the following responsibilities:

• PROJECT MANAGEMENT: Oversee the entire project management plan and all dependencies; coordinate changes resulting in consequences to all timelines and deliverables. Meet microsurgery deadlines and produce meaningful, accurate reports on the outcomes of the laboratory work.
• COMMUNICATION & COORDINATION: Coordinate communication between the Laboratory PI and contributing faculty/staff.
• COMPLIANCE: Oversee and ensure compliance requirements are met, including IRB, data use agreements, incident reports, and informatics systems compliance requirements.
• REPORTING AND DELIVERABLES: Oversee applicable reporting and deliverables; coordinate with all relevant teams to ensure deliverables related to their area of expertise and prepared and provided
• DATA COLLECTION: Collaborate with the PI and laboratory staff to ensure accurate and timely data collection and reporting. Follow prescribed protocols for data collection; ensure other study/project participants meet data collection needs.
• INFORMATICS AND DATA MANAGEMENT: Oversee the transition of study data to information management systems; collaborate to ensure data quality and control; ensure all required data are submitted according to guidelines and specifications. May manage one or more phase(s) of a major project(s) or all activities of a small project. May ensure overall lab compliance with all associated rules and regulations.

Specific Responsibilities:

Acting as a technical expert or specialist in a particular field within a research lab, establishes and optimizes protocols and coordinates conduct of multiple complex experiments.

Reviews results and revises protocols as necessary to ensure that project objectives are attained.

Molecular Biology:

• DNA, RNA, Protein isolation, purification and expression
• Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA
• Recombinant virus production and purification

Cellular Biology:

• Cell culture, cell isolation
• Functional studies
• Transfection

Histology:

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry
• Microscopy

Immunology:

• Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification

Animal:

• Husbandry
• Breeding and screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony

Administration

• Oversees and manages lab databases which may include ensuring that experiments are observed and that data is collected and entered correctly.
• Reviews and analyzes data.
• Creates computer models, graphs, reports and summaries for use in publications, professional journals, and grant applications.
• Co-authors scientific papers for presentation and publication.
• Research and obtains funding.
• Creates and maintains lab manuals regarding operating, safety, and etc. procedures.

Finance

• Creates lab financial plan and budget/audit expenses.
• Obtains sales quotes for lab equipment and supplies.
• Reviews and adjusts expenses to decrease costs.
• Oversees repair and maintenance of all lab equipment.
• Ensures that lab supplies are ready and available when necessary.

Supervision

• Trains, directs, assigns duties to and may supervise lab EEs, students, residents and/or fellows.

Miscellaneous

Performs other duties as assigned.

Minimum Qualifications:

• Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 5 years' lab/bench or other relevant experience required; OR
• Successful completion of a full course of study in an accredited college or university leading to a master's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years' lab/bench or other relevant experience.

Benefits:
At Northwestern, we are proud to provide meaningful, competitive, high-quality health care plans, retirement benefits, tuition discounts and more! Visit us at https://www.northwestern.edu/hr/benefits/index.html to learn more.

Work-Life and Wellness:
Northwestern offers comprehensive programs and services to help you and your family navigate life's challenges and opportunities, and adopt and maintain healthy lifestyles.
We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at https://www.northwestern.edu/hr/benefits/work-life/index.html to learn more.

Professional Growth & Development:
Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you're interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at https://www.northwestern.edu/hr/learning/index.html to learn more.

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