Scientist, Delivery Science

This role is considered On-Site.

Overview

The Delivery Sciences team is seeking a Scientist to contribute to our ligand discovery efforts for expanding RNAi to extrahepatic tissues. This position will focus on identifying, characterizing, and optimizing peptide and protein ligands for targeted delivery of RNAi therapeutics. This person will work closely with multiple groups across the research organization. The position is onsite and will be primarily located at 675 W Kendall St Cambridge MA.

Key Responsibilities

• Develop and optimize binders (peptide, VHH, mAb etc.) targeting conformationally diverse membrane proteins using molecular evolution techniques and affinity maturation strategies (e.g. phage display, yeast display, etc.)
• Characterize binders using a variety of biochemical techniques (ELISA, BLI/SPR, epitope binning) and in vitro assays (fluorescence microscopy, flow cytometry)
• Work collaboratively with multiple groups across the organization to evaluate ligands for tissue-specific delivery of RNAi in vitro and in vivo and enable them for development
• Design and execute well controlled experiments, analyze data, and present finding to various groups across the research organization

Qualifications

• PhD in biochemistry, biology, or a related field with 0-3 years of work experience in an academic or industry setting
• Expertise in peptide, scFv, and/or VHH library design, construction, and validation
• Expertise with identifying and optimizing binders targeting a broad range of proteins with high affinity using phage and/or yeast display and affinity maturation
• Experience with plasmid design for recombinant protein expression in bacterial and mammalian hosts
• Experience with preparative scale protein purification (IMAC, affinity, IEX, SEC) and standard recombinant protein characterization techniques (UV-vis, SDS-PAGE, Western blotting, ELISA)
• Experience with kinetic characterization of ligand/receptor interactions by BLI and/or SPR
• Strong interpersonal skills and excellent communication skills (written and oral) are required

About Alnylam:Alnylam Pharmaceuticals (Nasdaq: ALNY) has led the translation of RNA interference (RNAi) into a whole new class of innovative medicines with the potential to transform the lives of people afflicted with rare and more prevalent diseases. Based on Nobel Prize-winning science, RNAi therapeutics represent a powerful, clinically validated approach to treating diseases at their genetic source by "interfering" with mRNA that cause or contribute to disease. Since our founding in 2002, Alnylam has led the RNAi Revolution and continues to turn scientific possibility into reality.

Our culture:Our people-first culture is guided by our core values: fiercely innovative, open culture, purposeful urgency, passion for excellence, and commitment to people, and these values influence how we work and the business decisions we make. Thanks to feedback from our employees over the years, we've been fortunate to be named a top employer around the world. Alnylam is extremely proud to have been recognized as one of Science Magazine's Top Biopharma Employers, one of America's Most Responsible Companies for 2024 by Newsweek, a Fast Company Best Workplace for Innovators, and a Great Place to Work in Canada, France, Italy, Spain, Switzerland, and UK - among others.

At Alnylam, we commit to an inclusive recruitment process and equal employment opportunity. We are dedicated to building an environment where employees can feel that they belong, can bring their authentic selves to work, and achieve to their full potential. By empowering employees to embrace their unique differences at work, our business grows stronger with advanced and original thinking, allowing us to bring groundbreaking medicines to patients.